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Preservation Products / Coming Soon!
« Last post by Hooty Monroe on October 05, 2016, 02:42:39 PM »
                                                                                               PRESERVATION PRODUCTS

                                                                                                      COMING SOON!!!

 

There is a growing need to preserve and store biological specimens. Traditionally, this is accomplished by simple means, depending upon the characteristics and properties of the material being stored. Temperature is a key parameter for storing cells and tissues but typically storage of these materials involve some form of container (box, tube, etc.) that is kept at room temperature, refrigerated, or frozen.  We believe that our line of Preservation Products offer several unique advantages for the storage and preservation of biological materials.

Oxyrase® Preservation Products utilize the unique benefits of the Oxyrase® technology for an innovative approach to storage and preservation. Depending on your specific storage conditions, equipment, experiment or facility we offer the following products designed to store and preserve your bacteria, cells and other biological material. In all cases, the inclusion of the Oxyrase® Enzyme System provides a stable, reduced environment ideal for preservation.  Our preservation line includes:

    CryoBroth™ has been used by our internal QC and R&D teams for more than a decade to preserve and store our library of microorganisms.  This glycerol containing formulation is optimized for cryopreservation storage conditions (≤ -80°C)

    OxyStab™ is an exciting and convenient product, inspired by the need to preserve microorganisms in the laboratory.  This is a solid agar containing the Oxyrase® Enzyme System.  OxyStab™ is ideally used for refrigerated (2-8°C) and room temperature storage.

    OxyStasis™ is a revolutionary product that eliminates the damage to cells during frozen storage, eliminates the need for costly cryo-storage equipment and provides the convenience of preservation under normal freezer conditions (≤ -20°C)

 

To request additional information or to be included in product updates as we near the launch of these products please email sales@oxyrase.com.

                                                                                                           
                                                                                                             
 
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Upcoming Events / Spring SCACM 2015 Meeting!
« Last post by Hooty Monroe on February 11, 2015, 01:19:00 PM »
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Upcoming Events / Ohio 2014 Fall SCACM Meeting in Columbus, Ohio
« Last post by Hooty Monroe on October 13, 2014, 10:34:46 AM »
Please visit the Oxyrase, Inc. display at the 2014 Ohio Fall SCACM Meeting in Columbus, Ohio.
Learn how to increase your anaerobe recovery!


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33rd Annual Meeting of the Southwestern Association of Clinical Microbiology (SWACM) Fall 2014
                                  September 3 -6, 2014 in Houston, TX!
                       Congratulations to Lesa the winner of the Gift Basket!



 http://myemail.constantcontact.com/FALL-SWACM-2014-Meeting-in-Houston--TX.html?soid=1112636978526&aid=I7PVrohATAM
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POLL:
DO YOU SET BROTH BACK UP CULTURES TO RECOVER ANAEROBES FROM SPECIMENS?
COMMENTS ARE APPRECIATED!
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A question from one of our colleagues...

Is there a difference in the recovery of anaerobes under the following scenarios: When specimens are inoculated in Thio Broth? Or inoculated directly to PRAS media and then placed into anaerobic conditions?

1. When specimens are inoculated in Thio Broth?

    Historically, Thio Broth was the broth medium of choice as a back-up medium for recovering anaerobes from specimens. In general, broth has limitations for isolating anaerobes from
    specimens because most specimens for anaerobes contain mixed, multiple microorganisms. Both facultative microbes and anaerobes may be present. Thio Broth will support growth of both.
    Anaerobes may be found in higher numbers at the bottom of the broth tube. To be effective, Thio Broth must be heated to reduce the medium, then cooled before use. The Thio Broth tube
    serves as a source for possibly recovering the anaerobe, if they were not first recovered on a plate incubated anaerobically. Experience with Thio Broth as a back-up culture for recovering
    anaerobes has not been good. As a result, many microbiologists have discontinued its use.

2. Inoculated directly to PRAS media and then placed into anaerobic conditions?

    Use of PRAS broth media will enhance the recovery of anaerobes, but the same limitations described above would be operational. Whether the PRAS medium would make a significant
    difference in anaerobe recovery compared to Thio Broth is doubtful. The use of PRAS broth has the disadvantage of having to incubate the broth tube anaerobically. Thio Broth is incubated
    aerobically. Thio Broth would have a cost advantage.

3. There is a third alternative. Use OxyPRAS Broth and Oxyrase AnaSelect for Broth.

    First, the OxyPRAS broth is cell free, which eliminates complications found with other commercial broth media when doing gram stains on the broth culture. Second, Oxyrase AnaSelect for Broth
    creates anaerobic environments that eliminates use of a jar or anaerobic incubator. Third, Oxyrase AnaSelect for Broth is SELECTIVE for anaerobes and eliminates or retards growth of       
    facultative microbes. This powerful combination of attributes enhances the recovery of anaerobes from specimens.
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This is a study done by a 722 bed hospital in Mississippi comparing Oxyrase's OxyPRAS Plus Brucella and BBE/KVL Bi-Plates to their current PRAS Media.

To read more information on this comparison study and see the chart, please click the two attachments below.
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Answer:

The 'Pre' in pre-reduction refers to that time before the plate is made. Reduction is done at this step to prevent oxidized products, that are harmful to anaerobes, from being formed during sterilization (autoclaving) of the medium. Harmful oxidized products formed at this stage of media make-up cannot be removed later.

The 'Post' in post-reduction refers to that time after the plate is made. Reduction is done at this step to prepare the plate for use, i.e. inoculation with anaerobes. This is done to provide an optimal environment for the anaerobic, bacteria to initiate growth.

To make 'Pras' medium (pre-reduced, anaerobically sterilized) requires the pre-reduction step.

Some media 'made for anaerobes' only get the post-reduction treatment. A convenient way to visualize the recalcitrance of oxidized products to subsequent alteration is to imagine a rusty nail being put into an anaerobic environment... it does not reduce (remove/reverse) the rust... anymore than putting an oxidized plate, made without pre-reduction, into an anaerobic environment to convert it to a 'Pras' plate.
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Peggy from the Univ of Michigan is the WINNER
of the Gourmet Gift Basket at the Spring SCACM show 2014! Congratulations Peggy! Thanks for visiting the  Oxyrase, Inc. Booth!!

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Question: Difference between PRE-reduction & POST-reduction of anaerobe media?
 
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