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Topics - Hooty Monroe

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Preservation Products / Coming Soon!
« on: October 05, 2016, 02:42:39 PM »
                                                                                               PRESERVATION PRODUCTS

                                                                                                      COMING SOON!!!

 

There is a growing need to preserve and store biological specimens. Traditionally, this is accomplished by simple means, depending upon the characteristics and properties of the material being stored. Temperature is a key parameter for storing cells and tissues but typically storage of these materials involve some form of container (box, tube, etc.) that is kept at room temperature, refrigerated, or frozen.  We believe that our line of Preservation Products offer several unique advantages for the storage and preservation of biological materials.

Oxyrase® Preservation Products utilize the unique benefits of the Oxyrase® technology for an innovative approach to storage and preservation. Depending on your specific storage conditions, equipment, experiment or facility we offer the following products designed to store and preserve your bacteria, cells and other biological material. In all cases, the inclusion of the Oxyrase® Enzyme System provides a stable, reduced environment ideal for preservation.  Our preservation line includes:

    CryoBroth™ has been used by our internal QC and R&D teams for more than a decade to preserve and store our library of microorganisms.  This glycerol containing formulation is optimized for cryopreservation storage conditions (≤ -80°C)

    OxyStab™ is an exciting and convenient product, inspired by the need to preserve microorganisms in the laboratory.  This is a solid agar containing the Oxyrase® Enzyme System.  OxyStab™ is ideally used for refrigerated (2-8°C) and room temperature storage.

    OxyStasis™ is a revolutionary product that eliminates the damage to cells during frozen storage, eliminates the need for costly cryo-storage equipment and provides the convenience of preservation under normal freezer conditions (≤ -20°C)

 

To request additional information or to be included in product updates as we near the launch of these products please email sales@oxyrase.com.

                                                                                                           
                                                                                                             
 

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Upcoming Events / Spring SCACM 2015 Meeting!
« on: February 11, 2015, 01:19:00 PM »

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Upcoming Events / Ohio 2014 Fall SCACM Meeting in Columbus, Ohio
« on: October 13, 2014, 10:34:46 AM »
Please visit the Oxyrase, Inc. display at the 2014 Ohio Fall SCACM Meeting in Columbus, Ohio.
Learn how to increase your anaerobe recovery!



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33rd Annual Meeting of the Southwestern Association of Clinical Microbiology (SWACM) Fall 2014
                                  September 3 -6, 2014 in Houston, TX!
                       Congratulations to Lesa the winner of the Gift Basket!



 http://myemail.constantcontact.com/FALL-SWACM-2014-Meeting-in-Houston--TX.html?soid=1112636978526&aid=I7PVrohATAM

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POLL:
DO YOU SET BROTH BACK UP CULTURES TO RECOVER ANAEROBES FROM SPECIMENS?
COMMENTS ARE APPRECIATED!

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A question from one of our colleagues...

Is there a difference in the recovery of anaerobes under the following scenarios: When specimens are inoculated in Thio Broth? Or inoculated directly to PRAS media and then placed into anaerobic conditions?

1. When specimens are inoculated in Thio Broth?

    Historically, Thio Broth was the broth medium of choice as a back-up medium for recovering anaerobes from specimens. In general, broth has limitations for isolating anaerobes from
    specimens because most specimens for anaerobes contain mixed, multiple microorganisms. Both facultative microbes and anaerobes may be present. Thio Broth will support growth of both.
    Anaerobes may be found in higher numbers at the bottom of the broth tube. To be effective, Thio Broth must be heated to reduce the medium, then cooled before use. The Thio Broth tube
    serves as a source for possibly recovering the anaerobe, if they were not first recovered on a plate incubated anaerobically. Experience with Thio Broth as a back-up culture for recovering
    anaerobes has not been good. As a result, many microbiologists have discontinued its use.

2. Inoculated directly to PRAS media and then placed into anaerobic conditions?

    Use of PRAS broth media will enhance the recovery of anaerobes, but the same limitations described above would be operational. Whether the PRAS medium would make a significant
    difference in anaerobe recovery compared to Thio Broth is doubtful. The use of PRAS broth has the disadvantage of having to incubate the broth tube anaerobically. Thio Broth is incubated
    aerobically. Thio Broth would have a cost advantage.

3. There is a third alternative. Use OxyPRAS Broth and Oxyrase AnaSelect for Broth.

    First, the OxyPRAS broth is cell free, which eliminates complications found with other commercial broth media when doing gram stains on the broth culture. Second, Oxyrase AnaSelect for Broth
    creates anaerobic environments that eliminates use of a jar or anaerobic incubator. Third, Oxyrase AnaSelect for Broth is SELECTIVE for anaerobes and eliminates or retards growth of       
    facultative microbes. This powerful combination of attributes enhances the recovery of anaerobes from specimens.

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This is a study done by a 722 bed hospital in Mississippi comparing Oxyrase's OxyPRAS Plus Brucella and BBE/KVL Bi-Plates to their current PRAS Media.

To read more information on this comparison study and see the chart, please click the two attachments below.

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Peggy from the Univ of Michigan is the WINNER
of the Gourmet Gift Basket at the Spring SCACM show 2014! Congratulations Peggy! Thanks for visiting the  Oxyrase, Inc. Booth!!


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Question: Difference between PRE-reduction & POST-reduction of anaerobe media?
 

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To isolate and grow anaerobes, the literature teaches you need:
     An anaerobic environment, chemically reduced media...
    which is Pre-Reduced Anaerobically Sterilized


To read more on this comparison sheet click on the attachment below.

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A comparison of OxyPRAS Plus Brucella agar and media currently used for the general culture of anaerobes was performed here by Timothy R. Cassity, Ph.D., Clinical Microbiologist.

Please open the attachment below to view the chart.

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Media Comparison of OxyPRAS Plus plates vs. NonPRAS plates.
Data Collected from 794 bed Large hospital in OH in December 2012 utilizing an Anoxomat. Observations were made at 24, 48 and 72 hours.

Please click on the attachment below to look at the data chart.



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White Paper Library / PRAS Media White Paper: Anaerobe Microorganisms
« on: December 18, 2013, 03:15:00 PM »
Anaerobe microorganisms are among the most difficult microbes to isolate and grow. The environmental conditions required to grow anaerobes certainly contribute to these difficulties and add to the cost and time spent working with them in the clinical laboratory. http://

To read more information please click on PDF file.

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Today, clinical labs are increasingly adopting PRAS plates not only because they seek more reliable results that meet Best Practice criteria, but also because they are finding that manufactured PRAS plates are in a number of ways more reliable and economical.

To read more please click on the article link, Published in Bioscience Technology online.
 http://www.biosciencetechnology.com/articles/2013/01/deeper-look-pras-media-best-practices-isolate-and-grow-anaerobes-faster-and-more-reliably#.UswWn7R_ApA

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You may have need to recover an anaerobe from a lyophile. Your past experience may not have been always successful. This is not unusual. Some anaerobes are particularly sensitive to the lyophilization process, and the lyophile does not retain a high level of viability. The procedure used to recover the lyophilized anaerobe is your primary tool for obtaining viable microbes. 
 
In this White Paper, we provide step by step directions for recovering lyophilized anaerobes with an explanation as to why that procedure is followed.
 
If you have a mixed lyophile, containing a facultative microbe, recovery of the anaerobe becomes more difficult because the facultative microbe is likely to have survived the lyophilization process better than the anaerobe. At the start, the facultative microbe outnumbers the anaerobe. The situation gets worse. The facultative microbe may respond to the recovery procedure better than the anaerobe. Furthermore, the growth of the facultative microbe may out-pace the anaerobe, further complicating recovery of the anaerobe.
 
This White Paper provides a method for overcoming these difficulties and makes recovery of the anaerobe, separate from the facultative microbe, probable.


To read more information click on PDF file.

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